1. Presented by Rebecca Wingfield, M.D. and reviewed by Heba Mostafa, M.B.B.Ch., Ph.D.

A patient presents to labor and delivery for a C-section in the setting of fetal growth restriction and oligohydramnios. The anatomy ultrasound was unremarkable. The mother was rubella immune and negative for HIV, chlamydia, gonorrhea, and trichomonas on prenatal labs. Delivery was uncomplicated with clear amniotic fluids and Apgars were 8 and 9. The neonate was admitted to the NICU for low birth weight and the team would like to screen the infant for congenital cytomegalovirus (CMV) infection.

Which of the following sample types would be recommended for the diagnosis of congenital CMV?